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10 ml BSA solution was added to each sample and centrifuged at 300 g for 10 min followed by removal of the supernatant

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In double-blind, placebo-controlled trials, daily application of GHK-Cu cream at 3% concentration for 12 weeks produced a 70% increase in skin collagen thickness as measured by ultrasound imaging, a 40% increase in skin elasticity, and visible reduction in fine lines and wrinkles