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Higher peripheral concentrations of CRP have also been observed in individuals with BD compared to healthy volunteers, and these levels have been shown to decrease when symptoms remit (Fernandes et al., 2016)

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Agarose beads were separated through centrifugation, and the supernatant was added to a tube containing 1 μg of anti-His (Bethyl) antibody in the presence of Protein A/G agarose beads (InvivoGen) and incubated at 4 °C for 4 h